Open Access Research article

Identification of defensin-encoding genes of Picea glauca: characterization of PgD5, a conserved spruce defensin with strong antifungal activity

Pere Picart1*, Anna Maria Pirttilä2, Dora Raventos3, Hans-Henrik Kristensen3 and Hans-Georg Sahl1

Author Affiliations

1 Institut für Mikrobiologie und Biotechnologie, der Universität Bonn, Meckenheimer, Allee, Bonn, 167, 53115, Germany

2 Department of Biology, University of Oulu, Linnanmaa, Oulu, PO Box 3000 A6, FIN-90014, Finland

3 Novozymes A/S, Krogshoejvej, Bagsvaerd, 36, DK-2880, Denmark

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BMC Plant Biology 2012, 12:180  doi:10.1186/1471-2229-12-180

Published: 5 October 2012

Additional files

Additional file 1:

Alignment analysis of the deduced amino acid sequence ofendopiceasindiscovered by database searches. EST GQ0132.B7_K03 (endopiceasin) was found by screening the P. glauca EST database using the amino acid sequence of plectasin. The percentage similarity compared to plectasin is indicated in the last column.

Format: PPTX Size: 46KB Download file

Open Data

Additional file 2:

The complete coding sequence and the deduced amino acid sequence ofendopiceasinencoded within the EST GQ0132.B7_K03. The underlined amino acids represent the signal peptide, the amino acids in red indicate the pro-peptide while blue amino acids indicate the mature peptide.

Format: PPTX Size: 55KB Download file

Open Data

Additional file 3:

Alignment of the mature region of endopiceasin with other members of the defensins family. Alignment analysis of endopiceasin. The percentage similarity compared to endopiceasin is indicated in the last column. [Swiss-Prot:Q53I06.1] plectasin from fungi Pseudoplectania nigrella; [GenBank:BAB41027.1] defenisn A from arthropod Ornithodoros moubata; [GenBank:ABI52817.1] defensin B from arthropod Argas monolakensis. The six-cysteine residues are indicated by yellow and the disulphide bridge pattern is shown below.

Format: PPTX Size: 47KB Download file

Open Data

Additional file 4:

Alignment analysis ofPgD5genomic copies isolated from differentPiceasp. The differences between the sequences are indicated by yellow. The intron sequences are indicated in lowercase.

Format: PPTX Size: 89KB Download file

Open Data