Germination responses are affected in mutants of ATHB2, ATHB5 and At2g45210. Seeds of wild type and insertion lines were surface sterilised and, without stratification, germinated on 1xMS, supplemented with 0.01% ethanol (MOCK), 1, 2 or 5 μM Paclobutrazol (PAC), 1 or 5 μM abscisic acid (ABA). Germination was scored six days after imbibition. Seeds were deemed germinated if the radicle had visibly emerged from the seed coat. Statistical differences were analysed using a two-tailed t-test, and significant differences are indicated by one and two asterisks indicating p-values of p<0.05 and p<0.01, respectively.
Stamm et al. BMC Plant Biology 2012 12:179 doi:10.1186/1471-2229-12-179