Transcriptional activation by RGL2 involves promoter motifs bound by Dof-type transcription factors. Mesophyll protoplasts were transfected with reporter constructs carrying different promoter motifs 5’ of a minimal promoter (m35S) to drive GFP expression (B). Transfected protoplasts were treated with MOCK, DEX, or DEX plus GA3, and GFP signal was analysed by confocal microscopy (A). Signal intensities of 6 to 10 protoplasts of each construct/treatment-combination were determined using the Carl Zeiss LSM software, and plotted relative to the signal obtained from GFP driven by m35S only (C).
Stamm et al. BMC Plant Biology 2012 12:179 doi:10.1186/1471-2229-12-179