A mutation in RGL2 can rescue the non-germinating phenotype of the gibberellin-deficient mutant. Seeds of the double mutant ga1-3 rga-t2 fail to germinate, due to stabilised RGL2, whereas the mutation of RGL2 in the triple mutant ga1-3 rga-t2 rgl2-1 rescues germination to near wild-type levels. Germination rate was determined over a period of five days following stratification of seeds at 4°C for five days. Error bars indicated SD of three biological replicates. Seeds were deemed germinated when the radicle had visibly emerged from the seed coat. Pictures are representative of seeds/seedlings five days after imbibition.
Stamm et al. BMC Plant Biology 2012 12:179 doi:10.1186/1471-2229-12-179