Open Access Highly Accessed Research article

Insights into the molecular mechanism of RGL2-mediated inhibition of seed germination in Arabidopsis thaliana

Petra Stamm1, Pratibha Ravindran1, Bijayalaxmi Mohanty2, Ee Ling Tan1, Hao Yu13 and Prakash P Kumar13*

Author Affiliations

1 Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, 117543, Singapore

2 Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, 117576, Singapore

3 Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore, 117604, Singapore

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BMC Plant Biology 2012, 12:179  doi:10.1186/1471-2229-12-179

Published: 4 October 2012

Additional files

Additional file 1:

Genes differentially regulated in seeds of ga1-3 rga-t2 vs. ga1-3 rga-t2 rgl2-1. List of genes identified as RGL2-UP and RGL2-DOWN, respectively, with a fold-change of at least 2, and a p-value ≤0.01.

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Additional file 2:

Gene Ontology (GO) analysis of the RGL2-mediated transcriptome in seeds. GO analysis according to AgriGO (http://bioinfo.cau.edu.cn/agriGO/index.php), with respect to biological process (A, C) and molecular function (B, D) in RGL2-UP (A, B) and RGL2-DOWN (C, D).

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Additional file 3:

Cross-comparison of RGL2-mediated transcriptome in seeds with other available microarrays. List of genes differentially regulated by RGL2 in seeds that were identified in other available microarray data sets. Overlapping genes are indicated by ‘+’.

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Additional file 4:

Relative expression levels of ATHB2 and ATHB5 in response to abscisic acid. Seeds were stratified in water or 5μM abscisic acid, and RNA was extracted after 12h. Expression levels of ATHB2 and ATHB5 were determined by qRT-PCR in imbibed seeds of ga1-3 rga, relative to Tubulin (TUB), and compared to ga1-3 rga rgl2-1. RQ = relative quantity of transcript.

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Additional file 5:

Primers and oligonucleotides used. List of primers and oligonucleotides used for genotyping of T-DNA insertion lines, qRT-PCR, ChIP-qRT-PCR, and for construction of reporter constructs containing different promoter motifs.

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