Phenotypes induced by adominant negative FIL:SRDX construct. (A,B) 35SproI>>FIL plants grown on media without DEX (A) or with DEX (B). (C,D) 35SproI>>FIL:SRDX plants grown on media without DEX (C) or with DEX (D). (E) A fil yab3/FILpro::FIL plant displaying full complementation. Inset: fil yab3 double mutant plant. (F) fil yab3/FILpro::FIL:SRDX seedlings showing narrow cotyledons that are sometimes bifurcated (asterisk). Cotyledons of fil yab3 (G) and fil yab3 yab5 mutant seedlings (H). (I) fil yab3/FILpro::FIL:SRDX plant with needle-like leaves. (J) fil yab3 yab5 triple mutant plant with narrow and needle-like leaves. (K) Histochemical staining for YAB3:GUS activity in a fil yab3/FILpro::FIL:SRDX plant. YAB3 promoter activity is detected throughout young radial leaves. Inset: fil yab3/FILpro::FIL stained for GUS activity. (L) A fil/+ yab3/FILpro::FIL:SRDX plant with a fil yab3 mutant leaf phenotype. (M-R) Scanning electron micrograph showing the abaxial epidermis of wildtype (M), fil yab3 (N), fil yab3 FILpro::FIL (O) and fil/+ yab3/FILpro::FIL:SRDX (P) leaves. Note that the larger cell morphology in (N,P) is due to leaf adaxialisation. (Q,R) SEM showing epidermal cell morphology of fil yab3 yab5 needle leaves (Q) and those of fil yab3/FILpro::FIL:SRDX plants (R). Scale bars are 5 mm in (E,L) and inset in (A); 2 mm for (A-D, F-H); 1 mm for (I,J); 200 μm for (K) and inset in (K); 100 μm in (M-R).
Bonaccorso et al. BMC Plant Biology 2012 12:176 doi:10.1186/1471-2229-12-176