Table 3

Fusions of the right arms of chromosome 2 of tert/nbs1 and tert mutant Arabidopsis plants as revealed by FISH
tert/nbs1 tert
Gen. Line Anaphase bridges tested by FISH 2R signals 2R signals (%) Gen. Line Anaphase bridges tested by FISH 2R signals 2R signals (%)
G3 K12 36 26 72.2 G3 69-1 0 ND ND
G3 L2 41 0 0.0 G3 69-2 0 ND ND
G4 K12 40 18 45.0 G4 69-1 0 ND ND
G4 L2 45 3 6.7 G4 69-2 0 ND ND
G5 K12 32 27 84.4 G5 69-1 6 0 0.0
G5 L2 37 0 0.0 G5 69-2 5 0 0.0
G6 K12 42 33 78.6 G6 69-1 10 1 10.0
G6 L2 54 2 3.7 G6 69-2 12 0 0.0

Three sequential FISH with chromosome-end specific probes revealed enhanced occurrence of 2R chromosome ends in line K12 of the tert/nbs1 double mutants. In the sibling line K2, the fusion points were represented by random chromosome-arm interactions. In tert mutants lines 69–1 and 69–2, the occurrence of anaphase bridges starts from G5 on. Although the number of anaphase bridges available for analyses was rather low, cf. Figure 3G, only in one case the 2R-2R fusion had been detected. ND - no anaphases available.

Najdekrova and Siroky

Najdekrova and Siroky BMC Plant Biology 2012 12:167   doi:10.1186/1471-2229-12-167

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