Figure 5 .
The expression of genes putatively involved in the metabolism of secoiridoids and other phenolics compounds. The mRNA expression of genes putatively involved in the biosynthesis of terpenic (A) and phenolic moieties (B) of secoiridoids, in the biosynthesis of phenylpropanoids (C), in the degradation of phenolic compounds, and a schematic representation of their metabolism. The mRNA level was determined using RT-qPCR. GES: geraniol synthase; G10H: Geraniol 10-hydroxylase; NDHI: NADH dehydrogenase I; GT: SLS: Secologanin synthase; LAMT: Loganic acid methyltransferase; ADH: Arogenate dehydrogenase; CuAO: Copper amine oxidase; p-HPPA: p-hydroxyphenylpyruvic acid; p-HPAA: p-hydroxyphenylacetic acid; TYRD: Tyrosine/dopa decarboxylase; ALDH: Alcohol dehydrogenase; PPO: Polyphenol oxidase; PAL: Phenylalanine ammonia-lyase; and 4CL: 4-coumarate coenzyme A ligase. The relative mRNA levels are expressed as ΔΔCt. Bars = ±SE, n = 3. Different letters indicate significant differences between samples as determined using analysis of variance (Bonferroni’s post hoc tests, P < 0.05). Grey dotted box includes the secoiridoids compounds. Dotted arrows indicates uncertain biosynthetic steps.
Alagna et al. BMC Plant Biology 2012 12:162 doi:10.1186/1471-2229-12-162