Open Access Research article

Intraspecific sequence comparisons reveal similar rates of non-collinear gene insertion in the B and D genomes of bread wheat

Jan Bartoš1*, Čestmír Vlček2, Frédéric Choulet3, Mária Džunková2, Kateřina Cviková1, Jan Šafář1, Hana Šimková1, Jan Pačes2, Hynek Strnad2, Pierre Sourdille3, Hélène Bergès4, Federica Cattonaro5, Catherine Feuillet3 and Jaroslav Doležel1

Author Affiliations

1 Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Sokolovská 6, Olomouc, CZ-77200, Czech Republic

2 Institute of Molecular Genetics, Vídeňská 1083, Praha, CZ-14220, Czech Republic

3 INRA University Blaise Pascal UMR 1095 Genetics Diversity Ecophysiology of Cereals, Clermont-Ferrand, F-63100, France

4 INRA, National Resources Centre for Plant Genomics, Castanet Tolosan Cedex, F-31326, France

5 Instituto di Genomica Applicata, Via J. Linussio 51, Udine, 33100, Italy

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BMC Plant Biology 2012, 12:155  doi:10.1186/1471-2229-12-155

Published: 30 August 2012

Additional files

Additional file 1:

Table S1. List of EST and PCR primers used for BAC library screening and identification of contigs from target region.

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Additional file 2:

Table S2. Statistics of shotgun BAC clone sequencing and assemblies.

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Additional file 3:

Figure S1. 454 reads mapped to assembled sequences of the two BAC contigs

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Additional file 4:

Table S3. Promoter prediction for genes found at Hga locus at chromosome arm 3DS and at homoeologous region of chromosome 3B.

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Additional file 5:

Table S4. Analysis of expression for genes found at Hga locus at chromosome arm 3DS and at homoeologous region of chromosome 3B.

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