Open Access Research article

Temporal and spatial expression of polygalacturonase gene family members reveals divergent regulation during fleshy fruit ripening and abscission in the monocot species oil palm

Peerapat Roongsattham1, Fabienne Morcillo2, Chatchawan Jantasuriyarat3, Maxime Pizot1, Steven Moussu2, Dasuni Jayaweera4, Myriam Collin1, Zinnia H Gonzalez-Carranza4, Philippe Amblard5, James W Tregear1, Somvong Tragoonrung6, Jean-Luc Verdeil7 and Timothy J Tranbarger1*

Author Affiliations

1 Institut de Recherche pour le Développement, IRD Centre de Montpellier, IRD/CIRAD Palm Development Group, DIADE 911 avenue agropolis BP 64501, 34394, Montpellier cedex 5, France

2 Centre de Coopération Internationale en Recherche Agronomique pour le Développement, CIRAD, UMR DIADE, Montpellier, F-34398, France

3 Department of Genetics, Faculty of Science, Kasetsart University, Bangkhen Campus, 50 Phahonyothin Road, Jatujak, Thailand

4 Division, Loughborough, The University of Nottingham, Sutton Bonington Campus, School of Biosciences, Plant Science, Leicestershire, LE12 5RD, United Kingdom

5 PalmElit SAS, Montferrier-sur-Lez, F–34980, France

6 Genome Institute, National Center for Genetic Engineering and Biotechnology, BIOTEC, 113 Thailand Science Park, Phahonyothin Road, Klong 1, Klong Luang, Pathumthani, 12120, Thailand

7 Centre de Coopération Internationale en Recherche Agronomique pour le Développement CIRAD, UMR AGAP, MRI-PHIV, Montpellier, F-34398, France

For all author emails, please log on.

BMC Plant Biology 2012, 12:150  doi:10.1186/1471-2229-12-150

Published: 25 August 2012

Additional files

Additional file 1:

List of the 28 sequences that contain either a partial or complete GH28 PG signature domain.

Format: DOC Size: 73KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

Standard errors for Figure 3. Percentages were calculated from gene expression data derived from qPCR analysis that included individual values (3 technical repetitions) compared to the average expression of the reference gene (EgEF1α, elongation factor 1 α), together with the standard deviation (SD) for the following three tissue regions of the fruit: AZ, Abscission Zone; M, Mesocarp; P, Pedicel.

Format: DOC Size: 80KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 3:

Control experiments for in situ hybridization studies. Longitudinal sections of the fruit base were hybridized with digoxigenin-labelled RNA fragments of EgPG4 antisense (A) and sense (B), and the 18S ribosome antisense (C) and sense (D) probes after 6h ethylene treatment.

Format: PPT Size: 16.4MB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Open Data

Additional file 4:

Phylogenetic analysis of EgPG4, EgPG8 and EgPG10 with sequences from Arabidopsis and rice.

Format: PPT Size: 438KB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Open Data

Additional file 5:

List of primers used for expression analysis of oil palm PG genes by qPCR.

Format: DOC Size: 36KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 6:

List of primers used for the synthesis of in situ hybridation probes.

Format: DOC Size: 28KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data