Detection of miRNA-mediated mRNA cleavage using modified 5' RACE. Partial mRNA sequences from target genes were aligned with corresponding miRNAs. Each top strand (blue) represents a miRNA complementary site in the target mRNA, and each bottom strand (red) represents the miRNA. G:U wobble pairing (circles) and Watson-Crick pairing (vertical dashes) are indicated. Red arrows indicate the 5' termini of the degraded mRNA fragments isolated from Chinese fir, which were identified from cloned 5' RACE products, with the frequency of clones shown.
Wan et al. BMC Plant Biology 2012 12:146 doi:10.1186/1471-2229-12-146