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Open Access Highly Accessed Research article

Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes

Meng Ma12, Yan Yan12, Li Huang3, Mingshun Chen4 and Huixian Zhao12*

Author Affiliations

1 State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, Shaanxi, 712100, China

2 College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China

3 Department of Plant Sciences & Plant Pathology, Montana State University, Bozeman, MT59717, USA

4 USDA-ARS and Department of Entomology, Kansas State University, Manhattan, KS66506, USA

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BMC Plant Biology 2012, 12:141  doi:10.1186/1471-2229-12-141

Published: 10 August 2012

Additional files

Additional file 1:

Identity of the 176-bp fragment of1Bx14with the full-length ofHMW-GSgene1Ax1, 1Bx14, 1By15, 1Dx2and1Dy12in wheat cultivars Xiaoyan 6 and Shaanyou 225.

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Open Data

Additional file 2:

Nucleotide sequence alignment of theasXor theasYwith their corresponding elements in other x-type (upper region) or y-type (lower region)HMW-GSgenes expressed in the wheat variety, respectively;asXandasYrepresent sequences highly homologous to most of all x-type or y-typeHMW-GSgenes, respectively. Nucleotides conserved in all sequences are represented by ‘*’. The alignment was conducted using the Clustal W program.

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Open Data