Figure 4 .
Expression of DHNs in various organs of V. yeshanensis andV. vinifera. Total RNA was isolated from root (R), stem (St), leaf (L), seed (Sd) and fruit peel (P) at veraison, and was quantified using a Nanodrop spectrophotometer (Nanodrop Products, Wilmington, DE, USA). 1 μg DNase-treated total RNA was used as template for first-strand cDNA synthesis in a final volume of 20 μl, and subsequently 1 μl of this reaction was utilized for PCR amplification in a volume of 25 μl. Genomic DNA (D) was utilized as the positive control. RNA without reverse transcriptase was used as the negative control. The grapevine actin1 fragment was amplified as an internal control. 15 μl of PCR products were separated on a 1.5 % agarose gel containing ethidium bromide in each case.
Yang et al. BMC Plant Biology 2012 12:140 doi:10.1186/1471-2229-12-140