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Open Access Research article

CrMPK3, a mitogen activated protein kinase from Catharanthus roseus and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids

Susheel Kumar Raina, Dhammaprakash Pandhari Wankhede, Monika Jaggi, Pallavi Singh, Siddhi Kashinath Jalmi, Badmi Raghuram, Arsheed Hussain Sheikh and Alok Krishna Sinha*

Author Affiliations

National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Road, New Delhi, 110067, India

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BMC Plant Biology 2012, 12:134  doi:10.1186/1471-2229-12-134

Published: 7 August 2012

Abstract

Background

Mitogen activated protein kinase (MAPK) cascade is an important signaling cascade that operates in stress signal transduction in plants. The biologically active monoterpenoid indole alkaloids (MIA) produced in Catharanthus roseus are known to be induced under several abiotic stress conditions such as wounding, UV-B etc. However involvement of any signaling component in the accumulation of MIAs remains poorly investigated so far. Here we report isolation of a novel abiotic stress inducible Catharanthus roseus MAPK, CrMPK3 that may have role in accumulation of MIAs in response to abiotic stress.

Results

CrMPK3 expressed in bacterial system is an active kinase as it showed auto-phosphorylation and phosphorylation of Myelin Basic Protein. CrMPK3 though localized in cytoplasm, moves to nucleus upon wounding. Wounding, UV treatment and MeJA application on C. roseus leaves resulted in the transcript accumulation of CrMPK3 as well as activation of MAPK in C. roseus leaves. Immuno-precipitation followed by immunoblot analysis revealed that wounding, UV treatment and methyl jasmonate (MeJA) activate CrMPK3. Transient over-expression of CrMPK3 in C. roseus leaf tissue showed enhanced expression of key MIA biosynthesis pathway genes and also accumulation of specific MIAs.

Conclusion

Results from our study suggest a possible involvement of CrMPK3 in abiotic stress signal transduction towards regulation of transcripts of key MIA biosynthetic pathway genes, regulators and accumulation of major MIAs.

Keywords:
Catharanthus roseus; Methyl jasmonate; Mitogen activated protein kinase; Monoterpenoid indole alkaloid; Secondary metabolism