Open Access Research article

Sequence mining and transcript profiling to explore differentially expressed genes associated with lipid biosynthesis during soybean seed development

Huan Chen12, Fa-Wei Wang1, Yuan-Yuan Dong1, Nan Wang1, Ye-Peng Sun1, Xiao-Yan Li1, Liang Liu2, Xiu-Duo Fan2, Hai-Long Yin1, Yuan-Yuan Jing1, Xin-Yue Zhang1, Yu-Lin Li1, Guang Chen2* and Hai-Yan Li12*

Author Affiliations

1 Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin, 130118, China

2 College of Life Sciences, Jilin Agricultural University, Changchun, Jilin, 130118, China

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BMC Plant Biology 2012, 12:122  doi:10.1186/1471-2229-12-122

Published: 31 July 2012

Additional files

Additional file 1:

Table S1. Differentially expressed genes between 35 DAF and 15 DAF (FDR ≤0.001 and |log2ratio| ≥1).

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Additional file 2:

Table S2. Differentially expressed genes between 55 DAF and 15 DAF (FDR ≤0.001 and |log2ratio| ≥1).

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Additional file 3:

Table S3. Differentially expressed genes between 65 DAF and 15 DAF (FDR ≤0.001 and |log2ratio| ≥1).

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Additional file 4:

Table S4. Details of primers used for qRT-PCR.

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Additional file 5:

Figure S1. Scatter plot of differentially expressed genes in seeds harvested at 35 DAF, 55 DAF, and 65 DAF, compared with 15 DAF. (a). Scatter plot of differentially expressed genes between 35 DAF and 15 DAF. (b). Scatter plot of differentially expressed genes between 55 DAF and 15 DAF. (c). Scatter plot of differentially expressed genes between 65 DAF and 15 DAF. TPM = Transcript per million (normalized expression level of genes).

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Additional file 6:

Figure S2. Confirmation of differential gene expression by qRT-PCR.

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