Open Access Highly Accessed Research article

Global transcriptome analysis reveals distinct expression among duplicated genes during sorghum-Bipolaris sorghicola interaction

Hiroshi Mizuno1, Hiroyuki Kawahigashi1, Yoshihiro Kawahara1, Hiroyuki Kanamori1, Jun Ogata1, Hiroshi Minami2, Takeshi Itoh1 and Takashi Matsumoto1*

Author Affiliations

1 National Institute of Agrobiological Sciences (NIAS), Agrogenomics Research Center, 1-2, Kannondai 2-chome, Tsukuba, Ibaraki 305-8602, Japan

2 Mitsubishi Space Software Co. Ltd, Takezono 1-6-1, Tsukuba, Ibaraki 305-0032, Japan

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BMC Plant Biology 2012, 12:121  doi:10.1186/1471-2229-12-121

Published: 29 July 2012

Additional files

Additional file 1 :

Table S1. Expression ratios and ORF predictions of Phytozome transcripts.

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Additional file 2 :

Table S2. Expression ratios and ORF predictions of unannotated transcripts.

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Additional file 3 :

Table S3. Examples of differentially expressed novel transcripts.

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Additional file 4 :

Table S4. Examples of expression ratios and ORF predictions of differentially expressed transcripts.

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Additional file 5 :

Figure S1. Expression of genes associated with secondary metabolism of phenylalanine to naringenin. RPKMs of phenylalanine ammonia lyase (PAL), trans-cinnamate 4-monooxygenase (C4H), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI) are shown. PAL and CHS had tandemly duplicated putative paralogs. Figure S2. Expression of genes associated with target leaf spot infection. RPKMs of F3′Hs, ds1 LRR-RK, polyphenol oxidase, and C4 photosynthesis genes are shown. F3′H and polyphenol oxidases had tandemly duplicated putative paralogs that were differentially expressed.

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Additional file 6 :

Figure S3. Validation of expression level by quantitative real-time PCR (qRT-PCR). qRT-PCR of three biological replicates for each sample was performed and the means and standard deviations are shown. The expression level for each reaction was normalized against the expression level of the actin gene.

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Additional file 7 :

Table S5. Primers used.

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