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Open Access Research article

Characterization of a eukaryotic translation initiation factor 5A homolog from Tamarix androssowii involved in plant abiotic stress tolerance

Liuqiang Wang, Chenxi Xu, Chao Wang and Yucheng Wang*

Author Affiliations

State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), 26 Hexing Road, Harbin, China

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BMC Plant Biology 2012, 12:118  doi:10.1186/1471-2229-12-118

Published: 26 July 2012

Additional files

Additional file 1:

The promoter sequence of TaeIF5A1 and the cis-elements within the promoter. The cis-elements are shown in different colors and the PCR primers used for the amplification of promoter fragments used in the yeast one-hybrid assay are indicated by a solid line. The primers Pro-af and Pro-ar were used amplifying 461 bp promoter fragment, and Pro-bf and Pro-br were used amplifying 165 bp promoter fragment. The putative transcription start site is underlined and the start codon (ATG) is labeled with a rectangle.

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Additional file 2:

DNA and RNA gel blot analyses of TaeIF5A1-transformed poplars. A. Diagram of the T-DNA region of the pROKII-TaeIF5A1 vector used for transformation. B. DNA gel blot analysis of transformed plants. DNA (30 μg) from each sample was digested with BamH I and Sac I, separated on agarose gels, denatured and transferred to Hybond N+ membranes. C. RNA gel blot analysis of WT and the transgenic poplar plants. Total RNA (20 μg) from each sample was fractionated on formaldehyde agarose gel and blotted on Hybond N+ membranes. P, pROKII-TaeIF5A1 vector using as positive control; WT, wild type poplar plants; 1–10, ten lines of transgenic poplar plants.

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Additional file 3:

Primers used in the study.

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