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Open Access Methodology article

Improved efficiency of doubled haploid generation in hexaploid triticale by in vitro chromosome doubling

Tobias Würschum1*, Matthew R Tucker2, Jochen C Reif1 and Hans Peter Maurer1

Author Affiliations

1 State Plant Breeding Institute, University of Hohenheim, Stuttgart 70593, Germany

2 ARC Centre of Excellence for Plant Cell Walls, University of Adelaide, Waite Campus, Adelaide, Urrbrae SA 5064, Australia

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BMC Plant Biology 2012, 12:109  doi:10.1186/1471-2229-12-109

Published: 18 July 2012

Additional files

Additional file 1:

Table S1. Variance component analysis. Figure S1. Effect of in vitro chromosome doubling on embryogenesis. Results are shown for three different genotypes (F1 crosses) for the classical approach where the microspores are placed in Petri dishes directly after isolation, and for the approach presented here including an in vitro colchicine step (1 mM for 24h).

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