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Open Access Research article

Local and systemic changes in expression of resistance genes, nb-lrr genes and their putative microRNAs in Norway spruce after wounding and inoculation with the pathogen Ceratocystis polonica

Carl Gunnar Fossdal1*, Nadeem Yaqoob12, Paal Krokene1, Harald Kvaalen1, Halvor Solheim1 and Igor A Yakovlev1

Author Affiliations

1 Norwegian Forest and Landscape Institute, Høgskoleveien 8, As, NO-1431, Norway

2 Department of Ecology and Natural Resource Management, Norwegian University of Life Sciences, Høgskoleveien 12, As, NO-1432, Norway

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BMC Plant Biology 2012, 12:105  doi:10.1186/1471-2229-12-105

Published: 9 July 2012

Abstract

Background

NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus Ceratocystis polonica.

Results

The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes PaChi4, PaPAL and PaPX3 indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (PaLRR1, PaLRR3, PaLRR14, PaLRR15 and PaLRR16) showed significant increases greater than two-fold as a local response to C. polonica. Of all NB-LRRs only PaLRR3, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for miR3693 and miR3705.

Conclusions

Overall, local and systemic expression changes were evident only for the established resistance marker genes and PaLRR3. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.

Keywords:
qRT–PCR; Resistance; Ceratocystis polonica; Necrotroph; Picea abies; MicroRNA