Open Access Highly Accessed Research article

Identification of three MAPKKKs forming a linear signaling pathway leading to programmed cell death in Nicotiana benthamiana

Masayoshi Hashimoto, Ken Komatsu, Kensaku Maejima, Yukari Okano, Takuya Shiraishi, Kazuya Ishikawa, Yusuke Takinami, Yasuyuki Yamaji and Shigetou Namba*

Author Affiliations

Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

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BMC Plant Biology 2012, 12:103  doi:10.1186/1471-2229-12-103

Published: 8 July 2012

Additional files

Additional file 1:

Figure S1. Southern blot analysis of A) NbMAPKKKβ and B) NbMAPKKKγ using kinase domain-specific DNA probes. DNA probes were generated by using the PCR DIG Probe Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions. Each lane was loaded with 5 μg of total genomic DNA digested with each restriction enzyme.

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Additional file 2:

Figure S2. Ion leakage of GUS-infiltrated areas. An Agrobacterium strain expressing the GUS gene using the 35 S promoter was infiltrated at the following turbidities: 0.05, 0.5, and 1.0.

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