Identification of three MAPKKKs forming a linear signaling pathway leading to programmed cell death in Nicotiana benthamiana
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan
BMC Plant Biology 2012, 12:103 doi:10.1186/1471-2229-12-103Published: 8 July 2012
Additional file 1:
Figure S1. Southern blot analysis of A) NbMAPKKKβ and B) NbMAPKKKγ using kinase domain-specific DNA probes. DNA probes were generated by using the PCR DIG Probe Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions. Each lane was loaded with 5 μg of total genomic DNA digested with each restriction enzyme.
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