Open Access Research article

The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana

Jonathan Gilkerson123, Juan Manuel Perez-Ruiz34, Joanne Chory34 and Judy Callis12*

Author Affiliations

1 Department of Molecular and Cellular Biology, University of California, 1 Shields Avenue, Davis, CA, 95616, USA

2 Plant Biology Graduate Group, University of California, Davis, CA, 95616, USA

3 Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA

4 Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA

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BMC Plant Biology 2012, 12:102  doi:10.1186/1471-2229-12-102

Published: 8 July 2012

Additional files

Additional file 1: Figure S1:

Sequence of T-DNA borders in fln alleles. The T-DNA specific bands from PCRs were sequenced from the left border of the T-DNA for the three different lines. Orientation of the T-DNA is denoted by “L” for left and “R” for right borders. Boxed sequence is additional DNA not in T-DNA or at locus prior to insertion. Right border sequence at insertion site was not determined. Because fln2-1 and fln2-3 have two inverted T-DNA insertions in tandem, sequence on each side of the insertion could be determined. Unboxed sequence is present in endogenous locus.

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Additional file 2: Table S1:

DNA Primer sequences used for PCR. Contains a list of primer sequences, by purpose, used in this study.

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