Table 2

Transcriptional profiles of genes in Norton berry skin that are associated with secondary metabolism

ClusterA

Affymetrix ChipID

Genoscope ID

Function (VitisNet)B

KEGG

Pathway (VitisNet)


1

VVTU703_s_at

    GSVIVT00018175001

Phenylalanine ammonia lyase 2 (PAL2)

4.3.1.5

Phenylpropanoid

1

VVTU12705_s_at

    GSVIVT00024561001

Phenylalanine ammonia lyase (PAL)

4.3.1.5

Phenylpropanoid

18

VVTU26285_at

    GSVIVT00013936001

Phenylalanine ammonia lyase (PAL)

4.3.1.5

Phenylpropanoid

4

VVTU39693_at

    GSVIVT00008924001

Cinnamyl alcohol dehydrogenase (CAD)

1.1.1.195

Phenylpropanoid

6

VVTU2766_at

    GSVIVT00011484001

Sinapyl alcohol dehydrogenase (SAD)

1.1.1.195

Phenylpropanoid

10

VVTU14855_at

    GSVIVT00024588001

Cinnamyl alcohol dehydrogenase (CAD)

1.1.1.195

Phenylpropanoid

20

VVTU21888_at

    GSVIVT00011639001

Cinnamyl alcohol dehydrogenase (CAD)

1.1.1.195

Phenylpropanoid

2

VVTU13147_s_at

    GSVIVT00013987001

Cinnamoyl-CoA reductase (CCR)

1.2.1.44

Phenylpropanoid

7

VVTU12930_s_at

    GSVIVT00033763001

Cinnamoyl-CoA reductase (CCR)

1.2.1.44

Phenylpropanoid

12

VVTU3517_at

    GSVIVT00015738001

Cinnamoyl-CoA reductase (CCR)

1.2.1.44

Phenylpropanoid

13

VVTU914_at

    GSVIVT00038153001

Cinnamoyl-CoA reductase (CCR)

1.2.1.44

Phenylpropanoid

20

VVTU15680_at

    GSVIVT00020726001

Cinnamoyl-CoA reductase (CCR)

1.2.1.44

Phenylpropanoid

13

VVTU4884_at

    GSVIVT00002825001

Caffeoyl-CoA O-methyltransferase (CCoAOMT)

2.1.1.104

Phenylpropanoid

18

VVTU36108_at

    GSVIVT00025990001

Caffeic acid O-methyltransferase (CAOMT)

2.1.1.68

Phenylpropanoid

18

VVTU6966_s_at

    GSVIVT00026179001

Caffeate 3-O-methyltransferase 1 (COMT)

2.1.1.68

Phenylpropanoid

12

VVTU34546_at

    GSVIVT00009234001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU34913_at

    GSVIVT00007353001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU34551_x_at

    GSVIVT00031875001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU11765_at

    GSVIVT00004049001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU7619_x_at

    GSVIVT00005196001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU2775_x_at

    GSVIVT00007358001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU18886_x_at

    GSVIVT00007364001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU6035_x_at

    GSVIVT00009221001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

20

VVTU26310_s_at

    GSVIVT00031885001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

20

VVTU2671_at

    GSVIVT00009225001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

7

VVTU15752_at

    GSVIVT00002505001

Pinoresinol forming dirigent protein

DIRPR

Phenylpropanoid

16

VVTU8264_at

    GSVIVT00023306001

p-Coumaroyl shikimate 3'-hydroxylase isoform 1

K09754

Phenylpropanoid

14

VVTU25372_at

    GSVIVT00017649001

Ferulate 5-hydroxylase (F5H)

K09755

Phenylpropanoid

18

VVTU8974_at

    GSVIVT00036840001

Ferulate 5-hydroxylase (F5H)

K09755

Phenylpropanoid

14

VVTU34012_at

    GSVIVT00017653001

Ferulate 5-hydroxylase (F5H)

K09755

Phenylpropanoid

2

VVTU6513_s_at

    GSVIVT00038750001

Pinoresinol-lariciresinol reductase

PLR

Phenylpropanoid

15

VVTU15529_s_at

    GSVIVT00021542001

Secoisolariciresinol dehydrogenase

SIRD

Phenylpropanoid

20

VVTU2645_at

    GSVIVT00031383001

4-Coumarate-CoA ligase 2 (4CL)

6.2.1.12

Phenylpropanoid

1

VVTU17924_s_at*

    GSVIVT00014584001

Dihydroflavonol 4-reductase (DFR)

1.1.1.219

Flavonoid

12

VVTU14294_at

    GSVIVT00036313001

Dihydroflavonol-4-reductase (DFR)

1.1.1.219

Flavonoid

13

VVTU36178_s_at*

    GSVIVT00001063001

Leucoanthocyanidin dioxgenase (LDOX)

1.14.11.19

Flavonoid

11

VVTU9714_at

    GSVIVT00007249001

Flavonol synthase (FLS)

1.14.11.23

Flavonoid

13

VVTU33390_s_at

    GSVIVT00031249001

Flavonol synthase (FLS)

1.14.11.23

Flavonoid

14

VVTU13981_at

    GSVIVT00007247001

Flavonol synthase (FLS)

1.14.11.23

Flavonoid

18

VVTU2456_s_at

    GSVIVT00015347001

Flavonol synthase (FLS)

1.14.11.23

Flavonoid

10

VVTU16387_at

    GSVIVT00015842001

Naringenin,2-oxoglutarate 3-dioxygenase

1.14.11.9

Flavonoid

13

VVTU39787_s_at

    GSVIVT00036784001

Flavanone 3-hydroxylase (F3H)

1.14.11.9

Flavonoid

13

VVTU37475_at

    GSVIVT00037165001

Flavanone 3-hydroxylase (F3H)

1.14.11.9

Flavonoid

1

VVTU7778_at

    GSVIVT00034070001

Flavonoid 3-monooxygenase

1.14.13.21

Flavonoid

4

VVTU6932_at

    GSVIVT00016437001

Flavonoid 3-monooxygenase

1.14.13.21

Flavonoid

4

VVTU25410_s_at

    GSVIVT00036466001

Flavonoid 3-monooxygenase

1.14.13.21

Flavonoid

7

VVTU6362_at

    GSVIVT00017654001

Flavonoid 3-monooxygenase

1.14.13.21

Flavonoid

13

VVTU35884_at

    GSVIVT00022300001

Flavonoid 3',5'-hydroxylase (F3'5'H)

1.14.13.88

Flavonoid

10

VVTU13083_at*

    GSVIVT00005344001

Anthocyanidin reductase (ANR)

1.3.1.77

Flavonoid

13

VVTU9453_at

    GSVIVT00000479001

Quercetin 3-O-methyltransferase 1

2.1.1.76

Flavonoid

1

VVTU39820_s_at

    GSVIVT00037967001

Chalcone synthase(CHS)

2.3.1.74

Flavonoid

5

VVTU15193_at

    GSVIVT00003466001

UDP-glucose:flavonoid 7-O-glucosyltransferase (UFGT)

2.4.1.237

Flavonoid

14

VVTU22370_at

    GSVIVT00033493001

UDP-glucose:flavonoid 7-O-glucosyltransferase (UFGT)

2.4.1.237

Flavonoid

13

VVTU17578_s_at*

    GSVIVT00014047001

UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT)

2.4.1.91

Flavonoid

3

VVTU15110_at

    GSVIVT00001621001

Flavonol 3-sulfotransferase

2.8.2.25

Flavonoid

1

VVTU3684_s_at

    GSVIVT00029440001

Chalcone flavanone isomerase (CHI)

5.5.1.6

Flavonoid

17

VVTU563_at

    GSVIVT00020652001

Chalcone isomerase (CHI)

5.5.1.6

Flavonoid

10

VVTU9073_x_at

    GSVIVT00009968001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

2.4.1.238

Flavonoid

12

VVTU24324_at

    GSVIVT00024127001

Anthocyanidin 3-O-glucosyltransferase

2.4.1.115

Anthocyanin

18

VVTU35521_at

    GSVIVT00024993001

Anthocyanidin 3-O-glucosyltransferase

2.4.1.115

Anthocyanin

19

VVTU15768_at

    GSVIVT00037558001

Anthocyanidin 3-O-glucosyltransferase

2.4.1.115

Anthocyanin

20

VVTU14014_at

    GSVIVT00005849001

Anthocyanidin 3-O-glucosyltransferase

2.4.1.115

Anthocyanin

7

VVTU8698_at

    GSVIVT00008206001

Anthocyanidin rhamnosyl-transferase

RHATR

Anthocyanin

8

VVTU10613_at

    GSVIVT00026922001

Anthocyanidin rhamnosyl-transferase

RHATR

Anthocyanin

13

VVTU7774_at

    GSVIVT00011809001

UDP-rhamnose/rhamnosyltransferase

RHATR

Anthocyanin

5

VVTU8944_x_at

    GSVIVT00001860001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

RHGT1

Anthocyanin

12

VVTU14620_at

    GSVIVT00001853001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

RHGT1

Anthocyanin

16

VVTU15845_at

    GSVIVT00001851001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

RHGT1

Anthocyanin

17

VVTU15902_at

    GSVIVT00001859001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

RHGT1

Anthocyanin

18

VVTU36907_at

    GSVIVT00024130001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

RHGT1

Anthocyanin

3

VVTU5076_s_at

    GSVIVT00033502001

UDP-glucoronosyl/UDP-glucosyl transferase UGT75C1

UGT75C1

Anthocyanin

15

VVTU38572_at

    GSVIVT00025511001

CYP93A1 2-hydroxyisoflavanone synthase

1.14.13.86

Isoflavonoid

13

VVTU2075_at

    GSVIVT00019588001

CYP81E1 Isoflavone 2'-hydroxylase

1.14.13.89

Isoflavonoid

20

VVTU22627_at

    GSVIVT00019595001

CYP81E1 Isoflavone 2'-hydroxylase

1.14.13.89

Isoflavonoid

4

VVTU3973_at

    GSVIVT00026339001

2'-hydroxy isoflavone/dihydroflavonol reductase

1.3.1.45

Isoflavonoid

8

VVTU6973_at

    GSVIVT00003030001

Isoflavone methyltransferase

2.1.1.46

Isoflavonoid


A Clusters in bold exhibit steady increase of transcript abundance post véraison; Clusters in italics show decrease of transcript abundance post véraison. Expression profiling of each cluster is shown in Figure 1. B Function annotation and pathway assignment of each gene were based on VitisNet (http://vitis-dormancy.sdstate.org/pathways.cfm webcite). The genes (DFR, LDOX, ANR, UFGT) with asterisk have the same GSVIVT ID and display similar expression profiling as in qPCR.

Ali et al. BMC Plant Biology 2011 11:7   doi:10.1186/1471-2229-11-7

Open Data