Quantitative real-time (qPCR) assay of transcript abundance of the structural genes F3'H, F3'5'H1a, F3'5'H2a, DFR, LDOX, UFGT, ANR, LAR1, LAR2, GST and OMT in the flavonoid biosynthesis pathway during Vitis vinifera 'Cabernet Sauvignon' (blue dashed line) and V. aestivalis 'Norton' (red solid line) berry skin development. Cabernet Sauvignon berry skin were collected at 49, 59 (véraison, blue arrow), 71, 90 and 112 days after bloom (DAB), and Norton berry skin at 66, 71 (véraison, red arrow), 85, 99 and 127 DAB. Transcript abundance of each gene was normalized by the level of an actin gene. Bars indicate standard error of three biological replicates at each sampling time-point.
Ali et al. BMC Plant Biology 2011 11:7 doi:10.1186/1471-2229-11-7