Allele-specific transcription of cGAPDH. Frequency of allele-specific bases for three SNPs in PCR products from genomic DNA and from cDNA pools of small and large flower buds were obtained by pyrosequencing for the plants (H13, H19, H20) and are presented as boxplots consisting of sample minimum, lower quartile, median, upper quartile and sample maximum. Black boxes refer to genomic DNA, white boxes to cDNAs. Dotted lines represent the proposed frequency of an allele-specific base in genomic DNA and the null hypothesis of equal transcription for all alleles referring to their copy number: Alleles with one copy have an expected frequency of 0.2; alleles with two copies have an expected frequency of 0.4 and alleles with three copies have an expected frequency of 0.6 in pentaploids. Allele cGAPDH-1 has three copies in the plants H13 and H19 and two copies in H20, alleles cGAPDH-2 and cGAPDH-3 have one copy in all sampled plants. P-values of GLM statistics (additional file 2) comparing base frequencies of genomic and cDNA pools at a SNP are given above boxplots. Significant results are presented in bold. We did not find an allele-specific SNP for cGAPDH-4 suitable for pyrosequencing analysis, but sampled this allele in plant H20 from genomic DNA.
Ritz et al. BMC Plant Biology 2011 11:37 doi:10.1186/1471-2229-11-37