Relative expression levels of AOS-annotated transcripts in four chickpea varieties at different time points after salt stress onset, measured by qRT-PCR. The expression level of the INRA-93 derived 26 bp tag STCa-13267, annotated to an allene oxide synthase (AOS), was determined in the salt-tolerant chickpea variety (INRAT-93) and compared to less tolerant varieties. According to deepSuperSAGE, the AOS-annotated transcript is slightly (1,6-fold) down-regulated 2 h after stress, but the relative expression level remains very high when compared to the one of the salt-sensitive varieties. At subsequent time points, the AOS-expression decreases drastically in INRAT-93, whereas the levels in the salt-sensitive varieties remain constant.
Molina et al. BMC Plant Biology 2011 11:31 doi:10.1186/1471-2229-11-31