Analysis of KRP1 and 2 expression and CDK activity after NCS treatment. (A) qRT-PCR analysis of KRP1 and KRP2 expression relative to PP2A. (B) Immunoblot analysis of NCS-treated Arabidopsis roots using an anti-KRP2 antibody. Coomassie Brilliant Blue staining of the electrophoresis gel was used as a loading control. (C) P10CKS1At-associated kinase activity in NCS-treated roots. Autoradiogram showing typical results of CDK activity assays with histone as the substrate. Coomassie Brilliant Blue staining of the electrophoresis gel with histone was used as a control for equal substrate quantity per phosphorylation reaction. (D) Relative quantification of three independent kinase activity measurements as depicted in (C). The control was arbitrarily set at 100%. Data shown in (A) and (D) are means ± SD of three independent experiments.
Na et al. BMC Plant Biology 2011 11:184 doi:10.1186/1471-2229-11-184