Using a tobacco cell suspension to determine the effects of NCS on cell cycle. (A) The phenotype of 7 days after germination (DAG) tobacco seedlings grown on 0.5 μM NCS. Bar = 5 mm. (B) Primary root length of 7 DAG tobacco seedlings was inhibited by NCS. Values are means ± SD of three independent experiments (n ≧ 20). (C) Images of BY-2 cells treated with NCS and depletion of 2,4-D for 2 days. Bar = 50 μm. (D) Flow cytometric analysis of the cell cycle. (E) qRT-PCR detection of CYCD3;1, Histone H4, CYCA1;1 and CYCB1;1 mRNA after NCS-treatment in synchronized tobacco BY-2 cells. (F) E2Fa and RBR transcription level in BY-2 cells after NCS-treatment. The relative expression is normalized to ACTIN2. Data shown were the means ± S.D of three independent experiments.
Na et al. BMC Plant Biology 2011 11:184 doi:10.1186/1471-2229-11-184