Additional file 7.
Figure S6-Comparative proteomics studies demonstrating enzymes changes upon salinity stress. The total protein of soybean whole plant were extracted by TCA/Acetone methods followed by separation using 2-DE gels procedures (A). Three proteins (glutathione S-transferase, ascorbate peroxidase and dehydroascorbate reductase), which have a well documented involvement in the glutathione-ascorbate cycle and are closely associated with ROS elimination were chosen for further validation by image analysis (B). Besides, the GmGST was also verified by Western blot analysis (C). Consistent with the observations from 2-DE analysis, expression of GmGST, MDAR and APX were up-regulated in soybean plants.
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Wu et al. BMC Plant Biology 2011 11:178 doi:10.1186/1471-2229-11-178