TCL2 negatively regulates trichome formation on the inflorescence stems and pedicels. A. Expression of TCL2 in 35S:HA-TCL2 and 35S:TCL2RNAi transgenic plants. RNA was isolated form 10-day-old seedlings and RT-PCR was used to check the expression of TCL2. HA specific and gene specific primers were used to detect HA-TCL2, and gene specific primers were used to detect TCL2. The expression of ACTIN2 (ACT2) was used as a control. B. Glabrous phenotype in plant overexpressing TCL2 and ectopic trichome formation in TCL2 knockdown plants. From left to right: Rosette leaves (upper) and inflorescences (Lower) of Col, HA-TCL2, tcl1 and TCL2RNAi plants.
Gan et al. BMC Plant Biology 2011 11:176 doi:10.1186/1471-2229-11-176