Northern blotting. Northern blotting confirming differential expression of miRNAs. Total RNA (30 μg) from each of the four conditions (mock, drought, salinity, and alkalinity) was loaded and probed with miRNAs probe. The blot was hybridized with six miRNAs (miR166b, miR169d, miR482b, miR1507a, Gma-m001, and Gma-m002). The rRNA bands were shown as a loading control. miR166b, miR169d, and miR1507a expressed identical patterns when compared with Solexa sequencing.
Li et al. BMC Plant Biology 2011 11:170 doi:10.1186/1471-2229-11-170