Open Access Highly Accessed Research article

Characterization of the stress associated microRNAs in Glycine max by deep sequencing

Haiyan Li12, Yuanyuan Dong1, Hailong Yin2, Nan Wang1, Jing Yang1, Xiuming Liu12, Yanfang Wang12, Jinyu Wu13* and Xiaokun Li1*

Author Affiliations

1 Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin 130118, China

2 College of Life Sciences, Jilin Agricultural University, Changchun, Jilin 130118, China

3 Institute of Biomedical Informatics, Wenzhou Medical College, Wenzhou 325000, China

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BMC Plant Biology 2011, 11:170  doi:10.1186/1471-2229-11-170

Published: 23 November 2011

Additional files

Additional file 1:

Reads abundance of various classification of small RNAs. Reads abundance of various classification of small RNAs in mock and three stresses, drought, salinity, and alkalinity.

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Additional file 2:

The length size distribution of small RNAs. The length size distribution of small RNAs in mock, drought, salinity, and alkalinity, respectively.

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Additional file 3:

Known miRNAs identified in Glycine max. 133 known miRNAs corresponding to 95 miRNA families were identified in sequencing libraries of Glycine max under mock and three stresses.

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Additional file 4:

Frequency distribution of miRNA reads. Abundant miRNA reads frequency distributed in in mock and three stresses, drought, salinity, and alkalinity.

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Additional file 5:

Distribution of expressed miRNA tags. Number of expressed miRNA tags distributed in mock and three stresses, drought, salinity, and alkalinity.

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Additional file 6:

conserved miRNAs distributed in other species. miRNA sequences of soybean were conserved in other species.

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Additional file 7:

Prediction of novel miRNAs. Novel miRNAs identified from soybean.

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Additional file 8:

The predicted miRNA targeted genes in Glycine max.

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Additional file 9:

Novel miRNA targeted genes prediction in Glycine max.

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Additional file 10:

The sequences of antisense RNA probes.

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Additional file 11:

qRT-PCR primers of miRNAs.

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