Relative expression of VRNH3 and PPDH2. Detailed legend: Relative expression levels of VRNH3 and PPDH2 assayed by qRT-PCR in eight barley lines grown under different durations of vernalization and a short photoperiod: a) 15 d, b) 30 d, and c) 45 d. After vernalization, seedlings were subjected to no vernalization and a long photoperiod for 15 d. The results shown are normalized with respect to the level of the housekeeping gene Actin for each genotype and duration of vernalization. The variable of relative gene expression shown for each genotype and treatment is 2ΔCT, where ΔCT = CT Actin - CT target gene. Error bars represent the SEM. For each sampling time-point, bars with the same letter are not significantly different at P < 0.05 according to ANOVA that included all sampling time-points and genotypes per treatment.
Casao et al. BMC Plant Biology 2011 11:164 doi:10.1186/1471-2229-11-164