Research article
A highly conserved NB-LRR encoding gene cluster effective against Setosphaeria turcica in sorghum
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* Corresponding author: Tom Martin Tom.Martin@slu.se
1 SLU, Uppsala Biocenter, Dept. Plant Biology and Forest Genetics, Uppsala P.O. Box 7080, S-750 07, Uppsala, Sweden
2 Dept. of Crop Science, Makerere University P.O. Box 7062, Kampala, Uganda
3 National Agriculture Research Organisation, P.O. Box 295, Entebbe, Uganda
BMC Plant Biology 2011, 11:151 doi:10.1186/1471-2229-11-151
Published: 3 November 2011Additional files
Additional file 1:
PCR primer combinations used in cDNA-AFLP analysis.
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Additional file 2:
Maximum likelihood phylogenetic tree using the model JTT+G based on amino acid sequence from the coiled coil (CC), nucleotide binding (NB) and leucine rich repeat (LRR) domains of St proteins in sorghum, and closely related R proteins. Names refer to PHYTOSOME gene identifier. Physcomitrella patens R-protein Pp1s1_327V6, was used as an out-group. LR-ELW edge support values are shown [53]. Substitutions per site are indicated.
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Additional file 3:
Information on genes used in Figure 1 and their putative function. Data retrieved from the PHYTOSOME database. GenBank accession numbers are stated where present.
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Additional file 4:
Information on Arabidopsis genes used in Figure 3. Data retrieved from the TAIR database.
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Additional file 5:
Disease phenotypes on sorghum leaves, monitored 1-12 days post inoculation (dpi) with S. turcica on the resistant wild type GA06/18 and the susceptible Sila cultivar. The plants were treated with either water, empty BMV vector, construct 1 or construct 2, prior to fungal inoculation. The data is compiled from 25-30 plants per BMV construct and controls. The experiment was repeated 2 times.
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Additional file 6:
Gene specific primers for VIGS constructs. Restriction sites are in bold.
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Additional file 7:
List of primers used in real time PCR analysis.
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