Real time RT-PCR validation of the expression profiles of eight genes in the two seasons under analysis. Data are reported as means ± SE of three technical and two biological replicates. Transcript levels were calculated using the standard curve method and normalized against grapevine actin gene (VVTU17999_s_at) used as reference control. VVTU8069_at: L-galactono-1,4-lactone dehydrogenase (LGDH), VVTU12839_at: Arginine decarboxylase (ADC), VVTU16654_at: IMP dehydrogenase (IMDDH), VVTU39787_s_at: Flavonone- 3-hydroxylase (F3H), VVTU35625_s_at: Succinic semialdehyde dehydrogenase (SSADH1), VVTU1588_at: Ethylene receptor 1 (ETR1), VVTU9453: Quercetin 3-O-methyltransferase 1 (OMT1), VVTU4990_at: Gamma-glutamylcysteine synthetase (GCS).
Fortes et al. BMC Plant Biology 2011 11:149 doi:10.1186/1471-2229-11-149