Table 7

Expression analysis of common bean contigs.

454 Contig Number

2-ΔΔCT Values

Functional Annotation

Primer Sequences


Flower

Pod

Root


contig04711

3.43 ± 0.04

-2.20 ± 0.09

-0.82 ± 0.26

Alcohol dehydrogenase [Prunus armeniaca]

5'-ATA TGC CTC TGT CTT GGC AGG AGT-3'

5'-ACC TCG GGC AAT AGC ATT GAC TCT-3'

contig07734

2.16 ± 0.19

1.52 ± 0.07

-0.45 ± 0.1

Phosphoenolpyruvate carboxylase kinase [Glycine max]

5'-AGA ATG TGC GAA ACG CTG AAG ACG-3'

5'-AGG ATG GAA ACA CCG GAA GAT GGT-3'

contig08043

3.78 ± 0.18

-1.46 ± 0.17

-0.28 ± 0.18

Starch synthase III [Phaseolus vulgaris]

5'-AAG AAC TTG CTA GGG TGC AAG CTG-3'

5'-CTT TGC AGC TCT GTC TGC CTC AAT-3'

contig08830

-5.22 ± 0.14

6.91 ± 0.17

-1.24 ± 0.04

WRKY35 [Glycine max]

5'-TCA GCC TTG ACC TTG GTA TGG GAA-3'

5'-TTG CTG GTA TGA GCT TGG CTG TCA-3'

contig01300

*

-10.28 ± 0.07

1.32 ± 0.26

MADS box protein SEP3 [Lotus corniculatus var. japonicus]

5'-AAT TGC TCA TGC TTG GAC CTG CTG-3'

5'-TGA AGA CAT GGG ATA TGG CAG GCA-3'

contig13083

-0.69 ± 0.17

2.55 ± 0.12

1.49 ± 0.11

4-hydroxyphenylpyruvate dioxygenase [Glycine max]

5'-TTA TGC CAA CCT TCA CAA GCG TGC-3'

5'-TGC CCT GAT CGT CTC TGT CAA CAA-3'

contig14749

6.66 ± 0.08

0.17 ± 0.09

3.07 ± 0.27

Senescence-associated nodulin 1A [Glycine max]

5'-TTC TTC TTC CCT GCA CAC GAC ACT-3'

5'-TTG CTG CCC TTT CTA CGG ACA AGA-3'

contig17174

-1.41 ± 0.24

-6.58 ± 0.07

3.63 ± 0.12

Dihydroflavonol-4-reductase 2 [Glycine max]

5'-TGG TAG CCT CAT GCG AAC AGC ATA-3'

5'-AGG CCA GTT CGT GCA CTT AGA TGA-3'

contig14217

-1.88 ± 0.14

9.86 ± 0.1

1.15 ± 0.04

NAC domain protein NAC1 [Phaseolus vulgaris]

5'-TGG GTG CCC TTC CTT GAT AGA ACA-3'

5'-TGC AAC AGG GTT ACG CAC AAA TCG-3'

contig20010

6.07 ± 0.05

0.36 ± 0.08

1.34 ± 0.27

ABC transporter family protein [Arabidopsis thaliana]

5'-ACA ACC TTT GTT TCA GCA CGG AGC-3'

5'-GAG ACA TGG GCA ACT CAT TTG GCA-3'

contig28207

1.40 ± 0.19

-2.44 ± 0.12

-2.38 ± 0.1

Late embryogenesis abundant protein Lea14-A, putative [Ricinus communis]

5'-TGA CAG TCT GTT CTC CGT GTG CAT-3'

5'-TAA AGA ACC CAA ATC CGG TGC CGA-3'

contig28548

*

-1.99 ± 0.06

-0.33 ± 0.1

nodulin-26

5'-TTG GTC CAG GTC CAG CTA ACA ACA-3'

5'-CCC ATC GCC ATT GGT TTC ATC GTT-3'

contig29672

1.51 ± 0.24

3.16 ± 0.11

1.01 ± 0.07

glutathione S-transferase GST 19 [Glycine max]

5'-AGC TCT TCA AGG ACA CTG AGC CAA-3'

5'-AAA GGC TGT GGA TGC TGC ACT AGA-3'

contig28742

-0.84 ± 0.21

-6.13 ± 0.07

-4.19 ± 0.1

Fructokinase-like protein [Cicer arietinum]

5'-TGA GTA TTT GCT GAC GCG CTT CCT-3'

5'-GCA CAC CTG AAG GCA ATG GAA GTT-3'

contig28845

-0.98 ± .014

0.51 ± 0.1

-2.96 ± 0.04

NAC domain protein [Glycine max]

5'-TGG TGT GGT CCT GCA GAG TGT AAA-3'

5'-AAC GTC GGT GAT TGG GAG GAG AAA-3'

contig28932

4.83 ± 0.24

4.73 ± 0.07

3.81 ± 0.07

Nodule-enhanced protein phosphatase type 2C [Lotus japonicus]

5'-AAC GTC GGT GAT TGG GAG GAG AAA-3'

5'-CTT GCT GCT TCG CTT TGT CAC TGT-3'

contig28932

-4.04 ± 0.16

7.80 ± 0.12

-5.34 ± 0.12

nodule-enhanced protein phosphatase type 2C [Lotus japonicus]

5'-AAC GTC GGT GAT TGG GAG GAG AAA-3'

5'-ATC CCT CTC TCC TTC GCA GCA AAT-3'

contig30192

1.61 ± 0.22

*

0.58 ± 0.11

WRKY54 [Glycine max]

5'CAA CAC ACA CAT CCA AGC CCA GTT3'

5'TGG TTC TGC TGC TGC TGA TAC TGT3'

contig30958

1.17 ± 0.15

11.91 ± 0.11

3.89 ± 0.08

WRKY27 [Glycine max]

5'ACG GAA ACT CTG AGA GCA GCT CAA3'

5' TGC TTC CGT CCT CAC GTA AAC TCT3'

contig32565

-8.41 ± 0.15

-10.63 ± 0.11

0.90 ± 0.37

MADS transcription factor [Glycine max]

5'-TGC CTC ACC TAG CAA GTG TTC CTT-3'

5'-AGA TCT TGG CCC TCT AAG CAG CAA-3'

contig32665

1.26 ± 0.24

4.39 ± 0.09

0.33 ± 0.07

Transcriptional factor NAC11 [Glycine max]

5'-AAT GTG GTC TGA GGA GGT GGT GTT-3'

5'-ATG CTC TAA CTT CAG CGG AGG CAA-3'

contig32781

1.33 ± 0.17

1.82 ± 0.12

-2.89 ± 0.12

WRKY23 [Glycine max]

5'GCA TGT TGC TGT CAG GGT CAA TGT3'

5'TGG TGC TGA AGC TGA AAG TGT TGC3'

contig33251

-0.09 ± .019

-3.93 ± 0.06

-4.10 ± 0.1

Sucrose synthase [Vigna angularis]

5'-ACG GCT AGT TTC CTT GTG GGA GAA-3'

5'-TCT CAC ACA GCT TTC ACC CTT CCT-3'


* Data not available (inadequate replicates)

Kalavacharla et al. BMC Plant Biology 2011 11:135   doi:10.1186/1471-2229-11-135

Open Data