Figure 4.

Experimental validation of 48 common bean 454-sequencing derived unigenes by RT-PCR. Lanes with 50 bp ladder are lanes 1, 20, 21, 40, 41, and 60; Confirmation of absence of DNA contamination is shown in lanes 2-5 where RT-PCR amplification was carried out with primers designed from contig11286 in lanes with genomic DNA, leaf cDNA, leaf cDNA control (no reverse transcriptase added to reaction), and water as template to check DNA contamination. In lanes 6-19, 22-39, and 42-56, 58 and 59 RT-PCR products derived by amplification from an additional 47 common bean unigenes using leaf cDNA as a template are shown (complete list of contigs shown in Table 4). Lane 57 is amplification by the cons7 primers.