Effects of calcium ions on electrophoretic mobility of olive pollen caleosin. Effects of calcium ions on electrophoretic mobility of caleosin isolated from pollen OBs (A) and pollen coat (B). Proteins were extracted from pollen OBs and pollen coat and pre-treated with EGTA. Ten μg of proteins were incubated with CaCl2 (left lane), CaCl2 followed by EGTA (middle lane), or both CaCl2 and EGTA simultaneously (right lane). Immunoblots were probed with a FL anti-Clo3 Ab, followed by an anti-rabbit IgG Alexa 633-conjugated secondary Ab. Arrows show caleosin bands. Protein markers (kDa) are indicated on the left.
Zienkiewicz et al. BMC Plant Biology 2011 11:122 doi:10.1186/1471-2229-11-122