Additional file 5.

Western analysis of total proteins extracted from tassel branches containing anthers ranging from 1.0 to 3.0 mm in length, collected from homozygous am1-489 mutant (am1-489), homozygous am1-praI mutant (am1-praI), and wild type (WT) plants. Polyclonal antibodies raised to the AM1 protein and specific to actin (control) are described in Pawlowski et al. (10).

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Nan et al. BMC Plant Biology 2011 11:120   doi:10.1186/1471-2229-11-120