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Differential gene expression in incompatible interaction between wheat and stripe rust fungus revealed by cDNA-AFLP and comparison to compatible interaction

Xiaojie Wang1, Wei Liu1, Xianming Chen2, Chunlei Tang1, Yanling Dong1, Jinbiao Ma1, Xueling Huang1, Guorong Wei1, Qingmei Han1, Lili Huang1 and Zhensheng Kang1*

Author Affiliations

1 College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, PR China

2 USDA-ARS and Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA

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BMC Plant Biology 2010, 10:9  doi:10.1186/1471-2229-10-9

Published: 12 January 2010

Additional files

Additional file 1:

Two hundred fifty-five sequenced transcript derived fragments (TDFs) from Puccinia striiformis f. sp. tritici (pathotype CY23) infected wheat (cv. Suwon 11) leaves with altered expression patterns detected in the incompatible interaction using cDNA-AFLP and their closest matches in the GenBank database. containing Function group and TDF, Accession No., Size, Closest to database match, E-value, Expression and Primer pair. The number of TDFs in each category and the total number of TDFs are indicated in the parentheses and separated by "/".The TDFs marked with "*" were only found in the incompatible interaction when compared with the TDFs of the compatible interaction of the same wheat genotype, Suwon 11, with Pst pathotype CYR31 (See Reference No. [34]) using the BLASTN analysis; and those without "*" were common in the incompatible and compatible interactions.

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Additional file 2:

Selective primers used in cDNA-AFLP. displaying primers used for cDNA-AFLP.

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