Table 1

Results from eight TILLING assays in the B. rapa mutant population.

Gene name

EMS (%)

Length (bp)

GC (%)

Mutations detected

Screened M2 plants

Est. mutations per M2 plant*

M2 plants in population

Mutation density**

Expected mutations kb-1...


BraA.RPL.a

0.3

1007

47

21

768

13577 (10111)

6912

1/37 (1/49)

188 (164)

BraA.RPL.b

0.3

1072

45

149

4608

15082 (11730)

6912

1/33 (1/43)

209 (190)

BraA.RPL.c

0.3

1001

47

132

4608

14309 (10656)

6912

1/35 (1/47)

198 (173)

BraA.IND.a

0.3

1004

41

35

3072

5674 (4844)

6912

1/88 (1/103)

78 (78)

BraA.MET1.a

0.3

1104

48

94

3072

13858 (10105)

6912

1/36 (1/49)

191 (164)

BraA.MET2.b

0.3

1007

47

89

3072

14385 (10712)

6912

1/35 (1/47)

199 (173)


Relevant averages

12814 (9693)

1/44 (1/56)

177 (157)


BraA.RPL.b

0.4

1072

45

57

2304

11539 (8975)

2304

1/43 (1/56)

53 (48)

BraA.RPL.c

0.4

1001

47

40

2304

8672 (6458)

2304

1/358(1/77)

40 (35)


Relevant averages

10106 (7717)

1/51 (1/67)

47 (42)


* The estimated number of mutations per plant was calculated using an estimated genome size of 500 Mb for B. rapa [30]. Numbers in brackets were obtained after normalising to the average 35% GC content for the B. rapa genome [29].

** Mutation density based on estimated number of mutations per plant divided by the 500,000 kb

genome. Numbers in brackets were obtained after normalising to the average 35%

GC content for the B. rapa genome [29].

*** The number of expected mutations per kb in the population was obtained by extrapolating the

number of detected mutations in a subset of the M2 plants to the whole population.

Numbers in brackets were obtained after normalising to the average 41% GC content for B. rapa

exons [29].

Stephenson et al. BMC Plant Biology 2010 10:62   doi:10.1186/1471-2229-10-62

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