Distribution of mutations in the amplicons as a function of scaled fragment coordinates. The length of the TILLed fragments from BraA.RPL and BraA.IND.a genes were scaled to 1000 bp and the positions of each mutation calculated on this scale. The detected mutations are not equally distributed within the amplicons. Mutations are scarce close to the primers and appear more frequently towards the centre.
Stephenson et al. BMC Plant Biology 2010 10:62 doi:10.1186/1471-2229-10-62