Arabidopsis male meiocytes and anthers. A. A collection of anthers that undergo meiosis. In the buffer, the anther lobes should be clear and transparent (anther stages 5-7), otherwise it has passed meiosis and at free microscope stage (anther stages 8 or later). B. A thin section of a stage 6 anther. Male meiocytes were developing in the centers of four anther lobes (blue) with thick callose walls, and surrounded by tapetum (red) and the other anther wall cells (gray). C. A collection of male meiocytes. Male meiocytes clustered together in anther lobes in meiosis I and develop clear and thick callose-wall through late meiosis I and meiosis II, which are the indicators for distinguishing male meiocytes from somatic cells. Bars, 500 μm (A), 20 μm (B and C).
Chen et al. BMC Plant Biology 2010 10:280 doi:10.1186/1471-2229-10-280