High level transgenic expression of soybean (Glycine max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis pipeline
1 Department of Horticulture and Crop Science, OARDC/The Ohio State University, 1680 Madison Ave., Wooster, OH 44691 USA
2 Department of Biology, University of Virginia, Charlottesville, VA 22904 USA
3 Department of Microbiology, University of Virginia Health Systems, Charlottesville, VA 22908 USA
4 Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007 USA
5 USACE, Environmental Lab, ERDC, 3909 Halls Ferry Road, Vicksburg, MS 39180 USA
BMC Plant Biology 2010, 10:237 doi:10.1186/1471-2229-10-237Published: 4 November 2010
Although numerous factors can influence gene expression, promoters are perhaps the most important component of the regulatory control process. Promoter regions are often defined as a region upstream of the transcriptional start. They contain regulatory elements that interact with regulatory proteins to modulate gene expression. Most genes possess their own unique promoter and large numbers of promoters are therefore available for study. Unfortunately, relatively few promoters have been isolated and characterized; particularly from soybean (Glycine max).
In this research, a bioinformatics approach was first performed to identify members
of the Gmubi (
In this study, we present expression intensity data on 20 novel soybean promoters from two different gene families, ubiquitin and ERF. We also demonstrate the utility of lima bean cotyledons and soybean hairy roots for rapid promoter analyses and provide novel insights towards the utilization of these expression systems. The soybean promoters characterized here will be useful for production of transgenic soybean plants for both basic research and commercial plant improvement.