Figure 1.

The seu cyp85A2 double mutants condition enhanced gynoecial defects. Photomicrographs of indicated genotypes: panels A-L, flowers where some sepals and petals have been removed to allow viewing of gynoecia; panel M, rosette morphology. A) Col-0 wild type flower. B) seu-1 mutant flower. C) seu-3 mutant flower. Note slight split at gynoecial apex (arrowhead). D) sum63 single mutant flower. E) cyp85A2-2 mutant flower. F) cyp85A2-1. Gynoecium splitting is not detected in D-F. G) seu-1 sum63 double mutant. H) seu-3 cyp85A2-2 double mutant. I) seu-3 cyp85A2-1 double mutant. Enhanced splitting at the gynoecial apex is detected in the seu cyp85A2 double mutants relative to the respective single mutants. J-L) higher magnification of gynoecial apices shown in A, C and H, respectively. M) Rosette phenotypes. N-P) Nomarski optical images of chloral hydrate cleared stage 7 or early stage 8 gynoecia. N) In seu-3 the medial domain (md) extends to apex of gynoecium. At this stage the seu-3 mutant gynoecium shown is indistinguishable from wild type (not shown). O) In the seu-3 cyp85A2-1 double mutant the extent of the medial domain is reduced. P) Severely effected seu-3 cyp85A2-1 gynoecium. Adaxial portions of the medial domain are very reduced resulting in a "hollowed out" gynoecium. Scale bars in A is 1 mm for panels A-I; scale bar in J is 1 mm for images J-L; scale bar in M is 5 cm; scale bar in N is 0.1 mm for N-P.

Nole-Wilson et al. BMC Plant Biology 2010 10:198   doi:10.1186/1471-2229-10-198
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