Northern blot analysis of genes isolated from the RindPdigS library in control, wounded or P. digitatum -infected 'Navelina' oranges. RNA samples were obtained from either control, wounded or P. digitatum-infected 'Navelina' oranges at different times after inoculation. In brackets it is indicated whether the clone is a singleton or the cluster it belongs to. Membranes were hybridized with probes corresponding to: P0468B07.6 [O. sativa] (N03A05), beta-carotene hydroxylase (N03B01), 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (N03H08), Cytochrome P450 79A1 (N04D08), embryo-abundant protein (N04D11), nectarin 5 (N04D12), Caffeic acid 3-O-methyltransferase 1 (N04G08), tropinone reductase (N08F02), nectarin 5 (N09B03), caffeine synthase (N12F02), GcpE (N13A02), cellular apoptosis susceptibility protein (N13E10), aquaporin (N13F07), cystinosin (N13G07), hypothetical protein AN0433.2 (N14G01), homogentisic acid geranylgeranyl transferase (N14H09), wax biosynthesis protein (N17A02) and acetyltranferase-like protein (N17B07). Hybridization with the C. sinensis 26 S rDNA is shown in the bottom panel. Normalization of hybridization signals was carried out with respect to the hybridization signal of the C. sinensis rDNA. Values below the panels show the relative quantification of the corresponding hybridization signal referred to the value of the infected sample at 24 hpi. Those hybridization signals lower than two folds the intensity of background were not assessed (-).
González-Candelas et al. BMC Plant Biology 2010 10:194 doi:10.1186/1471-2229-10-194