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This article is part of the supplement: 15th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

Open Access Meeting abstract

Investigation of the PtdIns(4,5)P2 dependence of plasma membrane receptor endocytosis in living cells

Dániel Tóth, László Hunyady and Péter Várnai*

Author Affiliations

Department of Physiology, Semmelweis University, Faculty of Medicine, 1082 Budapest, Hungary

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BMC Pharmacology 2009, 9(Suppl 2):A50  doi:10.1186/1471-2210-9-S2-A50


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2210/9/S2/A50


Published:12 November 2009

© 2009 Tóth et al; licensee BioMed Central Ltd.

Background

Phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) plays an important role in various cellular processes: not only in calcium signalling as a precursor for the second messenger Ins(1,4,5)P3, but also in the regulation of ion channels, cytoskeletal dynamics and many other events connected to the plasma membrane. Since many of the molecules participating in the process of endocytosis can bind PtdIns(4,5)P2, a role of this lipid in the regulation of the internalization of plasma membrane receptors seemed possible.

Methods

In this study we focused on the investigation of the lipid dependence of the internalization of plasma membrane receptors, and we used the highly sensitive method of bioluminescence resonance energy transfer (BRET), which allows the detection of molecular closeness between two proteins labeled by bioluminescent and fluorescent markers. By fusing various plasma membrane receptors (e.g. angiotensin II AT1 receptor, serotonin 5-HT2C receptor and EGF receptor) to Renilla luciferase and applying YFP-tagged proteins as components of the endocytic machinery (β-arrestin, clathrin, β-adaptin, PM-targeted YFP, Rab proteins) we could follow the process with high temporo-spatial resolution in HEK cells. To decrease the plasma membrane PtdIns(4,5)P2 level we used the previously developed rapamycin-induced heterodimerization system, in which PtdIns(4,5)P2 depletion was achieved by the recruitment of 5-phosphatase enzymes to the plasma membrane.

Results

To check whether the PtdIns(4,5)P2 depletion was sufficient we measured the BRET signal between the PH domain of PLCδ1 - which binds specifically to PtdIns(4,5)P2 - fused to either Renilla luciferase or YFP. To follow receptor internalization we measured the BRET ratio between the receptors and plasma membrane-targeted YFP, which decreased upon stimulation with the appropriate agonist. After optimizing our system we were able to show that the internalization of EGF receptor was significantly reduced after depletion of the lipid, and the same was noticed in the case of AT1 and 5-HT2C receptors.

Conclusion

These data suggest that PtdIns(4,5)P2 level is an important factor in the regulation of plasma membrane receptor endocytosis.