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This article is part of the supplement: 15th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

Open Access Open Badges Meeting abstract

A single neurotoxic dose of MDMA decreases BDNF expression in the frontoparietal cortex but not in the hippocampus

Szilvia Vas1, Diána Kostyalik1, Csaba Ádori1, Rómeó D Andó2 and György Bagdy1*

Author Affiliations

1 Institute of Pharmacodynamics, Semmelweis University, 1089 Budapest, Hungary

2 Institute of Experimental Medicine, Hungarian Academy of Sciences, 1450 Budapest, Hungary

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BMC Pharmacology 2009, 9(Suppl 2):A41  doi:10.1186/1471-2210-9-S2-A41

The electronic version of this article is the complete one and can be found online at:

Published:12 November 2009

© 2009 Vas et al; licensee BioMed Central Ltd.


The popular recreational abuse drug 3,4-methylenedioxymethamphetamine (MDMA, 'Ecstasy') produces acute and long-lasting deficits in several markers of the serotonergic (5-HT) system. BDNF (brain derived neurotrophic factor) is a prominent trophic factor of serotonergic fibers. The aim of this study was to characterize the damage of serotonergic fibers in the frontoparietal cortex and hippocampus in parallel with the expression of BDNF.


Male Dark Agouti rats (7 weeks old) were treated with a single (15 mg/kg, i.p.) dose of MDMA. Density of tryptophan hydroxylase (TpH) immunoreactive (ir) fibers was quantified 3 days and 3 weeks after treatment. BDNF protein and mRNA levels were measured by ELISA and quantitative PCR method 24 hours and 3 weeks after treatment.


Findings from TpH-ir fiber density revealed significant (30-50%) decrease, compared to baseline, both 3 days and 3 weeks after treatment in both hippocampus and the frontoparietal cortex. Results from ELISA showed that BDNF level in the frontoparietal cortex dropped significantly (30%) 24 hours and 3 weeks after treatment. This decrease in BDNF concentration was also confirmed with the quantitative PCR method. In contrast, no such a reduction was found in the hippocampus.


Our findings show that administration of a single dose of MDMA, a neurotoxic agent, leads to a significant drop in the TpH-ir axon density with a parallel decrease in BDNF levels 3 days and 3 weeks after treatment in the frontoparietal cortex. In contrast, in the hippocampus, the reduction of TpH-ir axon density is not accompanied by the decrease of BDNF level. These results highlight regional differences in the expression of BDNF, the main neurotrophic factor of serotonergic fibers, in the terminal serotonergic areas after a neurotoxic dose of MDMA.


Supported by the EC, LSHM-CT-2004-503474.