Titration of germline tumor growth suppression by FUDR, resveratrol and pterostilbene. (A) Representative images of DAPI-stained glp-1(q485) animals treated for four days with 100 μM or 400 μM doses of FUDR, resveratrol and pterostilbene. The inhibitory effect of FUDR and pterostilbene on tumor size is particularly evident near the central region of the animal where the reflexed arms of the distal gonad meet (arrow in (a), (c) and (e)). (a, h) vehicle-treated controls. (h) is from the same experimental series as (c); (a) is from the same experimental series as other images; (b, c), FUDR at 100 μM (b) and 400 μM (c); (d, e), pterostilbene at 100 μM (d) and 400 μM (e); (f, g) resveratrol at 100 μM (f) and 400 μM (g). Scale bar = 250 μM. (B) Summary of results from three independent experiments for each compound at indicated doses. Each concentration was tested in at least 3 independent trials and a total of 36–100 animals were measured per compound dose in all experiments. Significance was judged by a paired t-test (2-tailed); *, #, ^ p < 0.05 versus untreated for FUDR-, pterostilbene- or resveratrol-treated animals, respectively.
Wilson et al. BMC Pharmacology 2008 8:15 doi:10.1186/1471-2210-8-15