Table 1

Endogenous polyamine and aminoglycoside-mediated inhibition of anthrax lethal factor activity-comparison with other proteases-(Mean ± SEM Math or IC50 values)

LF
 Bot
 MMP-9
 Furin
Name
 Math μM
 IC50 μM
 Math μM
 Math μM
Neomycin B tris-sulfate
 0.71 ± 0.04
 92.5
 >300
 N/T
Sisomicin Sulfate
 1.8
 >300
 >300
 N/T
Spermine, diphosphate salt
 0.9 ± 0.09
 57
 >300
 N/T
Amikacin
 23.3
 >300
 >300
 N/T
Neamine (free base)
 31.1 ± 5.6
 175
 >300
 N/T
Spermidine
 >100
 76
 N/T
 N/T
Apramycin
 111
 >300
 >300
 N/T
Putrescine (1,4-diaminobutane)
 >300
 >300
 >300
 N/T
Ac-CRATKML-N
 >300
 3.5
 N/T
 N/T
GM 6001
 7.2 ± 1.76
 >300
 0.002 ± 0.001
 N/T
H-RRRRRR-OH
 0.24
 N/T*
 N/T
 0.06
Ac-RRRRRR-OH
 0.29 ± 0.04
 N/T
 N/T
 0.05
Ac-RRRRRR-NH2
 0.12
 N/T
 N/T
 N/T
H-(D-Arg)-(D-Arg)-(D-Arg)-(D-Arg)-(D-Arg)-(D-Arg)-NH2
 0.04 ± 0.02
 N/T
 N/T
 0.06
H-R(NO2)R(NO2)
 >300
 N/T
 N/T
 N/T
Neomycin B hexaguanyl hexatrifluoroacetate salt
 0.03
 14
 >170
 1.5
Tetraguanyl neamine, free base
 0.3
 N/T
 N/T
 N/T

*N/T = not tested

Goldman et al. BMC Pharmacology 2006 6:8   doi:10.1186/1471-2210-6-8