Figure 9.

Effect of SP600125 on LPS and SB220025 stimulated NO production and iNOS mRNA expression. (A) Cells were stimulated with LPS 1 h before addition of tested compounds. 24 h after addition of LPS the nitrite concentrations were measured as marker of NO production. Values are mean ± S.E.M. (n = 6). (B) Cells were stimulated with LPS 1 h before addition of tested compounds. Incubations were terminated 10 h after LPS and extracted total RNA was subjected to real time RT-PCR. iNOS mRNA levels were normalized against GAPDH. Values are mean ± S.E.M. (n = 4). *P < 0.05, ***P < 0.001 compared with cells treated with LPS only.